Calculate
Print
Clear
AZ-UPLC-1
Method
Method Number:
Greenness Score:
Instrument Energy Score:
Solvent Energy Score:
Solvent EHS Score:
Technique:
-
HPLC
LC-MS
NPLC
Prep LC-MS
Prep SFC
SFC
SFC-MS
UPC2
UPLC
Number of analytes of interest:
Number of injections/runs for one full analysis:
Instrument Conditions
Flow Rate (mL/min):
Run time (min/injection):
Gradient
Time (min)
%A
%B
Time (min) 1
%A 1
%B 1
Time (min) 2
%A 2
%B 2
Time (min) 3
%A 3
%B 3
Time (min) 4
%A 4
%B 4
Time (min) 5
%A 5
%B 5
Time (min) 6
%A 6
%B 6
Time (min) 7
%A 7
%B 7
Time (min) 8
%A 8
%B 8
Time (min) 9
%A 9
%B 9
Time (min) 10
%A 10
%B 10
Mobile Phases
Mobile Phase A
Solvent 1
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 2
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 3
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Mobile Phase B
Solvent 1
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 2
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 3
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Sample
Sample Diluent
Sample prep volume (mL):
Number of sample preps:
Solvent 1
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 2
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 3
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Standards
Stock Standard Diluent
Stock standard prep volume (mL):
Number of stock standard preps:
Solvent 1
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 2
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 3
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Standard Diluent
Working Standard prep volume (mL):
Number of stock standard preps:
Solvent 1
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 2
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 3
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
SST
SST Diluent
SST prep volume (mL):
Number of SST preps:
Solvent 1
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 2
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 3
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Sensitivity
Sensitivity Soin Diluent
Sensitivity Soin prep volume (mL):
Number of Sensitivity Soin preps:
Solvent 1
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 2
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent
Solvent 3
-
Acetone
Acetonitrile
CO2
DMA
DMF
DMSO
Ethanol
Ethyl acetate
H2 (from generator)
Helium
Heptane (n)
Hexane (n)
Isopropanol
Methanol
NMP
Tetrahydrofuran
Water
Percent